MitoTool is a powerful web-based platform designed for analyzing mitochondrial DNA (mtDNA) sequences, offering essential tools for researchers in various fields. Whether it’s helping molecular anthropologists trace human evolution, assisting population geneticists in studying genetic diversity, supporting forensic scientists with mtDNA analysis in criminal investigations, or enabling biomedical researchers to identify pathogenic mutations, MitoTool provides critical insights across disciplines. Its user-friendly interface and unique capabilities make it an invaluable resource for advancing scientific discovery in these diverse areas of study.