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Bovine Transmembrane protein 68 (TMEM68) ELISA Kit

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Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q0VCR6
Abbreviation : TMEM68
Alternative Names : FLJ32370; MGC87778;
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?8.6%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TMEM68 in samples. An antibody specific for TMEM68 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM68 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TMEM68 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM68 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TMEM68 is a transmembrane protein (TP) protein that goes from one side of a membrane thro µgh to the other side of the membrane. Many TPs function as gateways or "loading docks" to deny or permit the transport of specific substances across the biological membrane, to get into the cell, or out of the cell as in the case of waste byproducts. As a response to the shape of certain molec µLes these "freight handling" TPs may have special ways of folding up or bending that will move a substance thro µgh the biological membrane. A transmembrane protein is a polytopic protein that spans an entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, altho µgh some of them (beta-barrels) can be also extracted using denaturing agents.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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