MitoTool is revolutionizing the way researchers approach mitochondrial DNA (mtDNA) analysis. With its intuitive, web-based platform, it allows scientists in fields like molecular anthropology, population genetics, forensics, and biomedical research to seamlessly analyze mtDNA sequences. By providing tools for variant identification, haplogroup determination, and the detection of potentially pathogenic mutations, MitoTool is empowering researchers to make groundbreaking discoveries. Its ability to process data in batch mode and allow access without login requirements makes it a valuable, time-saving tool for studies on human evolution, genetic diversity, crime scene investigations, and disease research