Maltose Binding Protein / MBP-probe(R29.6) , CF647 conjugate , 0.1mg / mL

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial , insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part , eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP) , the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose , the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively , the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution , MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors._x000D_ _x000D_ Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_