MECA-79(MECA-79) , Biotin conjugate , 0.1mg / mL

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins , including sulfated ligands for CD62L (CD34 , GlyCAM-1 , Sgp200 , and a subset of MAdCAM-1). This set of antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1 , 3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues , chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with PNAd from mouse , human and many other species and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo. Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.